Reincarnated as an Imperial Prince

Things About to Get Serious

It took more than two hours to set up the equipments in Alexander's new lab. With everything in place, he wasted no time. He donned his lab coat, and eye protection and gloves for safety.

He hefted a sack of soil from the corner and placed it on the table, then he opened it and sifted through the contents. He plucked a handful of soil from the sack and rubbed it between his fingers. Streptomyces griseus is a common bacteria that is usually found in soil. In the year 1943, Streptomycin was discovered by American biochemists Selman Waksman, Albert Schatz, and Elizabeth Bugie in Streptomyces griseus. Streptomycin is the first antibiotic that is effective against tuberculosis. They also found out that S. griseus yield more than 20 antibiotics, including actinomycin, clavacin, streptothricin, grisein, neomycin, fradicin, candicidin, and candidin to name a few.

For the discovery of Streptomycin, Selman Walksman received a Nobel prize for Physiology or Medicine in 1952, and the streptomycin was listed on the World Health Organization's List of Essential Medicines as the most effective and safe medicines needed in a health system. Dropping the case of tuberculosis significantly in the 1960s. He famously quoted, "Out of earth shall come to thy salvation."

In this world, Alexander doesn't know who will discover the streptomycin. Will there be a parallel Selman Walksman? There's no way of finding that out. But his sister desperately needs the medicine in order for her to survive. So Alexander will introduce a new revolutionary medicine that won't be used in medical treatments for another thirty years.

There's not much to do on the first day so Alexander will just prepare the inoculum containing Streptomyces griseus which can be done in a simple way.

First, he scooped a handful of soil where he will extract the Streptomyces griseus. Since there are a lot of bacteria found in soil, he'll have to grow a colony of bacteria, locate a strain of S. griseus, isolate it, then multiply it by using Hockenhul medium.

Second, he prepared for the dilution. The equipment used is nutrient agar composing 5 grams of peptone, 5 grams of sodium chloride, 3 grams of beef extract, 15 grams of agar, and 1000 millimeters of distilled water. The glassware is; a conical flask, Petri dishes, 1ml pipette, and test tubes.

Fortunately, in this world's era, autoclaves were already introduced, saving him a lot of time sterilizing each of the instruments.

Then in the dilution process where a solution mixed with soil sample and distilled water is transferred from one test tube to another, Alexander finished the process for thirty minutes.

After that, Alexander used the pipette to transfer the solution mixed and nutrient agar in the test tubes to each petri dish.

With that, step one is finished. He just has to wait for about five to six days for them to grow.

Looking at his wristwatch, Alexander saw the time was already two a.m. He started to feel sleepy and since his job for the day was done, he called it a day and left the laboratory.

Outside the door, there was a sign of mounted. "Keep Out, Only Authorized Personnel Allowed!" With a picture of a biohazard symbol.

...

Six hours later.

Christina knocked at his door. "Brother, wake up! It's already 8 in the morning."

Her call was only met with a snore.

Christina sighed, he's usually up by this time.

"Brother!" She knocked on the door again.

She heard a grumble and then a soft thump from the bed.