AU! In a small Texas town named Meadowbrook, a Meadowbrook elementary school has a lot of pressure to win the Texas Backyard Football League state championship this year. And with lot of expectations in the small Texas town's youth population at Bradley Elementary School, the Bradley Tigers team leader, Sherman Peabody faces a lot of pressure on and off the field.

Sherman Peabody is the toy of a child named Trent, Trent's favorite toy, in fact. But when Trent leaves, all the toys come alive and start chatting with each other, and they're especially concerned because Trent's birthday party is today, and they're afraid that the new toys might overshadow them, even to the point of having them be given away. But Sherman is there to calm them down because, of course, he's Trent's favorite, or, at least, was. A new toy named Jimmy Neutron has all the latest gizmos and technology. Sherman inevitably gets jealous and tries to fight for his spot as alpha dog. Then, both Sherman and Jimmy are now lost. They must find a way to get back to Trent before he moves without them, but they will have to pass through a ruthless toy killer, Duncan Ryanson.

Jimmy, Sherman, and their friends are back as 10 year old Trent heads off to Camp Wauwanakua, leaving his toys to their own devices. Things shift into high gear when an obsessive toy collector name Evan Worthington, the son of the owner of Worthington's Toy Stadium, kidnaps Sherman. At the Worthingtons' apartment, Sherman discovers that he is a highly valued collectible from a 1990's TV show called "Nicktoons Roundup." He meets the other prized toys from the show: Penny Peterson, Cindy Vortex, Libby Fulfax, Sheen Eztavez, Carl Wheezer, the PS118 students, Gompers the goat, and Goten the Prospector. Trent's toys mount a daring rescue mission, Jimmy Neutron meets his match, and when he is tempted by the idea of lasting forever in a museum, Sherman has to decide where he and his heart truly belong.

Trent is now nineteen years old and is going to college. His mother presses him to decide the fate of his toys, and Trent decides to leave them in the attic, with the exception of Sherman and Penny that he intends to bring with him. However Trent's mother donates them by accident to the Lifetime Daycare. The toys are welcomed by and Timmy Turner but sooner they discover that the children mistreat them. Further, they are imprisoned in the daycare by Timmy and his gang. Now, all of the toys must band together in one final, crazy scheme to escape their confines and return home to Trent.