The mane 7 were transferred to New York City and meet three new friends Casey, April, and Karai and later meet the turtles who know that they have powers and fight together and the Dazzlings have return and it up to the girls to defeat them.

Twilight Sparkle is the princess of the Crystal Kingdom and is loved by her parents, but one day the Shredder and the Foot Clan came and try to take over her home and force Twilight to marry one of his guards, so her parents ask Master Splinter, master in the art of Ninjutsu of the Hamato Clan, to take her somewhere safe and was sent to earth to be protect. Then after 15 years, Twilight learns the truth and started to train her magic to defeat Shredder once and for all. Join Twilight Sparkle on excited adventures with new friends, new crushes, new enemies, and new magic.

In the land of Equestria where people have powers of each mages, Princess Celestia receives a letter from Hamato Yoshi, leader of the Hamato Clan and wants to make a peace treaty and unite Canterlot and The Hamato. But as days went by Hamato Yoshi's sons Leo, Raph, Donnie, and Mikey fell in love with Princess Celestia's faithful students Twilight Sparkle, Rarity, Sunset Shimmer, and Pinkie Pie and became friends. But Flash Sentry a traitor to Canterlot grew jealous that Twilight love Leo and Flash will do anything to get rid of Leo and take over Canterlot.

After defeating the shredder and his minions, the boys Leo, Donnie, Raph, and Mikey (they are human) with their sister Miwa (Tang Shen is still alive and the mother to the ninja boys and wife of Hamato Yoshi) and friends April and Casey move to a new city and go to a new school called Canterlot High and made new friends: Te Rainbooms, Twilight, Sunset, Rarity, Pinkie, Applejack, Fluttershy, and Rainbow Dash and begin to have a new life and starts to find love.