Yeast Two Hybrid

Yeast two-hybrid (Y2H) screening is the classical high throughput screening technology for the study of protein-protein interactions. It was first utilized by Stanley Fields and Ok-Kyu Song over 20 years ago. It is now still widely used for the initial identification of potential protein interactors. The major advantages of this technique including: 1. it is cost-effective as the technique does not require special equipments; 2. the screening is highly customizable and sensitive; 3. Y2H is an in-vivo assay, and yeast, as a eukaryotic organism, supports post translational modification and correct folding of eukaryotic proteins, which together enables discovery of physiologically relevant protein interactions.

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