E.coli Surface Display

The chimeric protein of Lpp and OmpA is the most frequently used carrier for bacterial surface display in Escherichia coli, but arrays of other anchors or carriers have also been employed, including PhoE, OmpC, TraT, FhuA, or intimin. In addition, gram-negative bacteria also assemble complex structures at their surfaces, such as outer membrane lipoproteins, ice nucleation protein, flagella, pili or S-layers, all of which have been used for display. With rich experience, Creative Biogene could help you determine the best surface-anchoring motif according to the sizes of passengers.