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cre expression plasmid are a kind of double-stranded, closed-loop DNA with a size range from 1kb to more than 200kb. All kinds of antibody expression plasmid are self-replicating genetic components that keep existing in the cytoplasm and can be independent of the cell chromosome.
Generally, they are in a free state outside the chromosome. But under certain conditions, they can also be reversibly integrated into the host chromosome. It replicates with the replication of chromosomes and then it can be transmitted to offspring through cell division. At present, There are many methods to be used to extract plasmid DNA. The method of alkaline lysis is used to extract plasmid DNA.