Banana wilt caused by Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4), is a worrying destructive banana disease of which there is not yet effective control measures. The present study aimed to evaluate the ability of earthworms Eisenia fetida to be biological control agents against Foc TR4. Methodological approach consisted of assessing interactions between Foc TR4 and (i) enzyme β-N-Acetyl-glucosaminidase (NAGase) and (ii) E. fetida extracts that are the coelomic fluid (CF) and the crude crushed (CC). Then NAGase were dosed in E. fetida CF. Foc TR4 growth was inhibited by NAGase but no effect was observed with the extracts CF and CC of E. fetida. Enzymatic dosage showed that CF contained 0.015 ± 0.006IU/mg protein as NAGase activity. These results suggest the possible use of E. fetida in biocontrol of Foc TR4 however through a process other than the extracts CC and CF. The outcomes of this study may constitute background data allowing to explore potential of earthworms in biocontrol of banana pathogenic fungi, which is of great significance to the development of banana industry system and to the reduction in the use of fungicides.