This paper aims to examine the isolation of Lactobacillus from dairy products (milk, curd, and yogurt), extraction of Bacteriocin from it as well as to determine their inhibitory effect against few fungal skin pathogens and bacterial skin pathogens such as: Candida albicans, Aspergillus sp., Malassezia sp., Fusarium sp., and Penicillium sp., Staphylococcus aureus, Streptococcus pyogens, Klebsiella sp. The antagonistic activity of Lactobacillus sp. is mainly due to the bacteriocin present in it, therefore in this study, the bacteriocin is extracted and checked against the indicated microorganisms. Kirby Bauer Disc Diffussion method is used and zone of clearance is observed around the pathogenic species indicating that they shows some kind of antagonistic activities which are further measured and noted, which gives a clear picture about the degree of resistance of bacteriocin against the pathogenic microorganisms.