The Aquarium & Shark Lab at Hendersonville, North Carolina, has been the center of global attention due to Charlotte, a female round stingray. For over eight years, Charlotte has lived alone in her tank, without any male stingrays, yet in a remarkable and puzzling twist, she became pregnant. This incredible story of the NC Aquarium pregnant stingray has captivated scientists and marine enthusiasts worldwide, with the aquarium even organizing live ultrasound sessions to provide insight into her unexpected pregnancy. However, Charlotte's journey took a surprising turn, shedding light on a rare reproductive disorder.