TMT Tandem Mass Tag Proteomics
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Ongoing, First published Oct 24, 2024
https://www.inomixo.com/services/tmt-tandem-mass-tag-proteomics.html

The tmt based quantitative proteomics technology is a relative and absolute quantification technique for peptide-based proteomics. It utilizes 6, 10, or 16 isotopic labels to specifically label the amino groups of peptides. Through tmt tandem mass tag spectrometry analysis, it enables the simultaneous comparison of protein expression levels across up to 16 different samples. TMT  labeling is a high-throughput screening technique widely used in quantitative proteomics research.

TMT based Proteomics Workflow
Uniform processing of samples labeled in the same batch reduces sample handling and experimental errors, resulting in high experimental reproducibility.

High quantitative stability is achieved when samples are labeled in the same batch.

The use of fractionation methods significantly increases the depth of protein identification.

TMT Proteomics Provides Distinct Advantages Over iTRAQ
Quantitative Accuracy:TMT Offers higher quantitative accuracy and precision due to improved isotopic purity and reduced interference.
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