Preliminary studies oMedicinal plants have wide medicament application used to prevent and management of many ailments. These plants are used for primary health care in human communities who are deprived of modern medical care. Urinary tract infections (UTI) are most common form of bacterial infections, affecting people throughout their lifespan. Hence the present study to evaluate the antibacterial potential of folk medicine against Urinary tract infection causing pathogens. In the present study 25 urine samples were collected and from the samples totally 58 bacterial colonies were isolated, among the 58 colonies six dominant urinary tract bacterial pathogens were identified by using Biochemical and Morphological characters which were confirmed as Escherichia coli, Klebsiella pneumoniae, Proteus vulgaris, Pseudomonas aeruginosa, Staphylococcus aureus and Streptococcus pyogenes. The antibacterial activity of folk three medicinal plant extract such as Allium sativum, Allium cepa and Zingiber officinale, used against bacteria isolates. Compared with folk three medicinal plant extract the Allium sativum was effective to kill the all bacteria isolates. Hence the herbal drugs have to be subjected to extensive pharmacological, toxicological and clinical tests to confirm the prescribed status. Thus the ethnobotanical approach will be like a search for molecular diversity subjecting a wide variety of new molecules from plant sources and testing them with as many different tests as possible.All Rights Reserved