BlueGene Biotech Mouse Endotoxin ELISA kit (E03E0134)

https://www.elisakit.cc/products/mouse-endotoxin-elisa-kit/

E03E0134 Mouse Endotoxin ELISA kit

The Mouse Endotoxin ELISA kit can be used to identify samples from the mouse species. Endotoxin can also be called Lipopolysaccharides, LPS, and EDT.

Principle of the Assay
EDT ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-EDT antibody and an EDT-HRP conjugate. The assay sample and buffer are incubated together with EDT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the EDT concentration since EDT from samples and EDT-HRP conjugate compete for the anti-EDT antibody binding site.