Moringa oleifera is a common plant and known for various medicinal properties. The research work was conducted to investigate the anticytotoxic and antioxidant activity of dried flower powder extract of leaf Moringa oleifera. The ethanol extracts from flower of moringa plants contain severalphytochemicals such asalkaloids,amino acids, quinones, cardiac glycosides, flavonoids, phenols, saponins, tannins, terpenoids, coumarins and triterpenoids.The antioxidant activities of different concentrations of ethanol extracts of the leaves were determined by the three assay techniques i.e., DPPH radical scavenging assay, Ferric reducing ability power (FRAP). The type of chemical bonds is identified through FTIR analysis. The results obtained in the present study indicate that the leaves of Moringa oleifera are a potential source of anticytotoxic and antioxidants.