When the Second Hokage degrades the Uchiha race as "possessed by evil", Sasuke doesn't take kindly to his clan being stereotyped by Tobirama, when the Uchiha were the people Sasuke had known firsthand as kind, good civilians, living and enjoying life the same as anyone else, the people he lived with and treasured as his comrades.
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It couldn't be clearer that Sasuke doesn't appreciate Tobirama's rude, uncalled-for stereotyping of his people, made evident in several ways, one being the death glare Sasuke levels at Tobirama for writing his entire race off as "evil", and another being Sasuke's hostile battle-readiness to go toe-to-toe with Tobirama if necessary.
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At this point, Sasuke is in control of his emotions, so he calmly inquires why Tobirama is so compelled to degrade Sasuke's entire people group as "possessed by evil", wanting to know what evidence Tobirama has to back his claim.
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