✦ 𝖗𝑒ɪ .

BABYYYYY

important concl about MM kinetics:
          
          1. Characteristic of Km
          - reflects affinity of E for that S
          - numerically = [S] at which V = 1/2 Vmax
          - Does not vary with [E]
          - low Km, high affinity of E for that substrate
          hight Km, low affinity of E for that substrate
          
          bcs high concen. of S is needed to half-saturate the E
          
          2. Rship of V to [E]
          ROR is directly proportional to [E] at all substrate concentration
          
          3. Order of rxn

MM EQN - describe how rxn velocity varies with substrate concentration
          
          Assumption made in deriving MM rate EQN:
          1. [S] is much greater than [E]
          2. Rate of formation of ES complex is equal to rate of dissociation/breakdown of ES complex -> E+S and E+P
          3. ROR is measured as soon as E and S are mixed that is initial reaction volecities (V0) are used

how enzyme works?
           
          1. Energy changes occuring during formatiom rxn
          - Free energy of activation = barrier that separates reactant and product - difference in energy between reactant and intermediate occurs during formation of product
          - ROR - Low Ea, high Ea because molecule has sufficient energy to pass through intermediate state
          - Alternate rxn pathway - allows for lower Ea, accelerate ROR - enzyme does not change Ea of reactant, products and even EQ of the rxn 
          
          2. Chemistry of active site
          - Transition state stabilization - Active site + substrate -> initiate conversion of substrate to transition state and accelerate ROR
          - Other mechanisms - active site provides catalytic groups that enhance transition state formation

enzyme : protein catalysts that increases ROR without being changed in over all process
          
          - oxidoreductase
          - lyases
          - ligases
          - hydrolase
          - isomerase
          - transferase

Miss u:(

haloo hruu

suka dho lesap setahun lepastu datang balik tiba tiba

anyone asking hows rei doing... shes good. doa la kuman tak suka mata dia (tiber)(tapi ni real ges)

ramai betul ketuk mb rei tapi dianya lesap dibadai kerasnya realiti