The Run Away Queen Of Hell

Chapter 6-Dr.Chua

 I am constantly fighting everything. Right now, it feels like I am in a never-ending battle with myself.

1. 1,4-dioxane: 3 out of the 6 marker families were impaired in liver cells, all 6 families were impaired in neuronal cells, especially the cell stress markers. This substance can lead to all 6 of the pathological pathways explored.

2. 2-butoxyethanol: 41 of the 51 genes present on our DNA chips were significantly repressed (for some of them, gene expression was reduced a hundred-fold). All studied cellular functions were severely compromised in both cell lines.

3. 3-aminophenol: 36 of the 51 genes were repressed, ten-fold for some of them. All studied cellular functions were compromised in both cell lines.

4. 4-aminobiphenyl: 4 genes were overexpressed in liver cells (markers for cell stress,DNA damage, cell cycle control and hormonal response) ; 25 genes were repressed in neuronal cells, in all 6 pathological pathways explored.

5. Abamectin: 44 of the 51 genes were strongly repressed, a hundred-fold in some cases. 27 genes were repressed in both cell lines. This insecticide (targeting GABAsynapses) has an unequivocal pathological potential.

6. Acetaminophen (paracetamol): 30 genes were repressed quite strongly, 2 genes were overexpressed, affecting all cellular functions under study in both cell lines.

7. Acetonitril: 2 genes were repressed and 3 overexpressed in liver cells (markers for cell stress, DNA damage, cell cycle control, neurotoxicity and hormonal response). 15 genes were repressed in neuronal cells, affecting all studied cellular functions.

8. Benzoic acid (E210): 38 of the 51 genes were repressed, significantly in some cases. All studied cellular functions were disturbed in both cell lines.

9. Acrylamide: 3 genes were overexpressed, 30 were repressed, significantly in some cases. In neuronal cells, the response was mainly observed after exposure to the highest dose. In contrast, liver cells were also affected at low doses. All studied functions were affected in both cell lines.

10. Aldicarb: expression of 20 genes was affected in both cell lines and a further 24 genes were affected in one or the other cell line, totalling 44 markers elicited. All studied cellular functions were affected in both cell lines.

11. Aldrin: 14 genes were affected in liver cells, mainly overexpressed. 11 genes were affected in neuronal cells, of which, 2 were dramatically overexpressed. All studied cellular functions were affected in both cell lines, with very different reactions for each gene.

12. Benzophenone-3: 2 genes were repressed (cell stress and neurotoxicity) and 1 overexpressed (DNA damage) in liver cells. 16 genes were repressed in neuronal cells, affecting all studied functions in this cell line.

13. Bisphenol A: 1 gene was repressed in liver cells after exposure to high dose. 7 genes were overexpressed in liver cells after exposure to low dose. 20 genes were repressed in neuronal cells, affecting all studied functions.

14. Carbaryl: 4 genes were overexpressed and 7 were repressed in liver cells, affecting all studied functions in this cell line. The effect was dramatic on neuronal cells, in which 48 genes were significantly repressed (a hundred-fold or more).

15. Chlorpyriphos: expression of 42 genes was affected in both cell lines and a further 7 genes were affected in one or the other cell line, totalling 49 markers elicited out of the 51 harboured by our DNA chips. Genes were repressed except for one neurotoxicity marker, which was overexpressed in neuronal cells.