Two-spirit is an exclusive gender for Native Americans. Typically two-spirit people fulfill one of many mixed gender roles found traditionally among many Native Americans and Canadian First Nations indigenous groups.
Traditionally the roles included wearing the clothing and performing the work of both male and female genders. The term usually implies a masculine spirit and a feminine spirit living in the same body and was coined by contemporary gay, lesbian, bisexual, and transgender Native Americans to describe themselves and the traditional roles they are reclaiming.
This English term emerged in 1990 out of the third annual intertribal Native American/First Nations gay/lesbian American conference in Winnipeg. The term can also be used more abstractly, to indicate the presence of two contrasting human spirits (such as Warrior and Clan Mother) or two contrasting animal spirits (which, depending on the culture, might be Eagle and Coyote).
The term berdache was coined by western anthropologists and used until the late 20th century, mainly to describe feminine Native Americans assigned male at birth. The term is however inaccurate and can nowadays be considered offensive. Non-natives who use this identity are often accused of "appropriating Native culture."
There's no recognized flag, but here are some of the designs
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