The Romanovs

Romanov resting place

In 1991, the presumed burial site of the imperial family and their servants was excavated in the woods outside Yekaterinburg. The grave had been found nearly a decade earlier, but was kept hidden by its discoverers from the Communists who still ruled Russia when the grave was originally found. The grave only held nine of the expected eleven sets of remains. DNA and skeletal analysis matched these remains to Tsar Nicholas II, Tsarina Alexandra, and three of the four grand duchesses (Olga, Tatiana and presumably Maria). The other remains, with unrelated DNA, correspond to the family's doctor (Yevgeny Botkin), their valet (Alexei Trupp), their cook (Ivan Kharitonov) and Alexandra's maid (Anna Demidova). Forensic expert William R. Maples decided that the Tsarevitch Alexei and Anastasia's bodies were missing from the family's grave. Russian scientists contested this conclusion, however, claiming that it was the body of Maria that was missing. The Russians identified the body as that of Anastasia by using a computer program to compare photos of the youngest grand duchess with the skulls of the victims from the mass grave. They estimated the height and width of the skulls where pieces of bone were missing. American scientists found this method inexact.

American scientists thought the missing body to be Anastasia because none of the female skeletons showed the evidence of immaturity, such as an immature collarbone, undescended wisdom teeth, or immature vertebrae in the back, that they would have expected to find in a seventeen-year-old. In 1998, when the remains of the imperial family were finally interred, a body measuring approximately 5'7" was buried under the name of Anastasia. Photographs taken of her standing beside her three sisters up until six months before the murders demonstrate that Anastasia was several inches shorter than all of them.

From left to right, Grand Duchess Olga Nikolaevna of Russia, Tsar Nicholas II, Grand Duchess Anastasia Nikolaevna of Russia and Grand Duchess Tatiana Nikolaevna of Russia in captivity at Tobolsk in the winter of 1917
In 1991, the presumed burial site of the imperial family and their servants was excavated in the woods outside Yekaterinburg. The grave had been found nearly a decade earlier, but was kept hidden by its discoverers from the Communists who still ruled Russia when the grave was originally found. The grave only held nine of the expected eleven sets of remains. DNA and skeletal analysis matched these remains to Tsar Nicholas II, Tsarina Alexandra, and three of the four grand duchesses (Olga, Tatiana and presumably Maria). The other remains, with unrelated DNA, correspond to the family's doctor (Yevgeny Botkin), their valet (Alexei Trupp), their cook (Ivan Kharitonov) and Alexandra's maid (Anna Demidova). Forensic expert William R. Maples decided that the Tsarevitch Alexei and Anastasia's bodies were missing from the family's grave. Russian scientists contested this conclusion, however, claiming that it was the body of Maria that was missing. The Russians identified the body as that of Anastasia by using a computer program to compare photos of the youngest grand duchess with the skulls of the victims from the mass grave. They estimated the height and width of the skulls where pieces of bone were missing. American scientists found this method inexact.[65]

American scientists thought the missing body to be Anastasia because none of the female skeletons showed the evidence of immaturity, such as an immature collarbone, undescended wisdom teeth, or immature vertebrae in the back, that they would have expected to find in a seventeen-year-old. In 1998, when the remains of the imperial family were finally interred, a body measuring approximately 5'7" was buried under the name of Anastasia. Photographs taken of her standing beside her three sisters up until six months before the murders demonstrate that Anastasia was several inches shorter than all of them.

Grand Duchess Anastasia Nikolaevna of Russia aboard the Rus, the ship that ferried her to Yekaterinburg in May 1918. This is the last known photograph of Anastasia.
The account of the "Yurovsky Note" indicated that two of the bodies were removed from the main grave and cremated at an undisclosed area in order to further disguise the burials of the Tsar and his retinue, if the remains were discovered by the Whites, since the body count would not be correct. Searches of the area in subsequent years failed to turn up a cremation site or the remains of the two missing Romanov children. However, on August 23, 2007, a Russian archaeologist announced the discovery of two burned, partial skeletons at a bonfire site near Yekaterinburg that appeared to match the site described in Yurovsky's memoirs. The archaeologists said the bones were from a boy who was roughly between the ages of ten and thirteen years at the time of his death and of a young woman who was roughly between the ages of eighteen and twenty-three years old. Anastasia was seventeen years and one month old at the time of the assassination, while her sister Maria was nineteen years, one month old and her brother Alexei was two weeks shy of his fourteenth birthday. Anastasia's elder sisters Olga and Tatiana were twenty-two and twenty-one years old respectively at the time of the assassination. Along with the remains of the two bodies, archaeologists found "shards of a container of sulfuric acid, nails, metal strips from a wooden box, and bullets of various caliber." The site was initially found with metal detectors and by using metal rods as probes.

DNA testing by multiple international laboratories such as the Armed Forces DNA Identification Laboratory and Innsbruck Medical University confirmed that the remains belong to the Tsarevich Alexei and to one of his sisters, proving conclusively that all family members, including Anastasia, died in 1918. The parents and all five children are now accounted for, and each has his or her own unique DNA profile. However, as reported in one of the studies:

It should be mentioned that a well publicized debate over which daughter, Maria (according to Russian experts) or Anastasia (according to US experts), has been recovered from the second grave cannot be settled based upon the DNA results reported here. In the absence of a DNA reference from each sister, we can only conclusively identify Alexei - the only son of Nicholas and Alexandra.