how a monoclonal antibodies used in pregnancy tests

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-Pregnant women produce HCG hormone in their urine

-Test strips has three parts to it open brackets first: start contains antibodies complementary to HCG, second: middle contains antibodies complemented HCG- antibody complex, third: and contains antibodies complementary to HCG antibodies]

-If woman is pregnant, HCG in the urine binds to the antibodies on the 1st part forming a HCG- antibody complex, the HCG- antibody complex then binds to antibodies on the second part forming a blue line [positive result], HCG antibodies also bind to the third part as a control.

-if woman is not pregnant, no HCG in urine so nothing binds to HCG antibodies in the first part, so nothing binds to antibodies in the second part leaving no blue line [negative result], the HCG antibodies still buy into the third part for the control. 

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