Lecture 17
Good morning. Good morning. I don't know about you, but I can't take too many more nights like this. I confess, I haven't gotten a thing done for so many nights in a row now, but what a game! How many of you saw the Lecture 17
Good morning. Good morning. I don't know about you, but I can't take too many more nights like this. I confess, I haven't gotten a thing done for so many nights in a row now, but what a game! How many of you saw the game? Excellent. Very good, very good. You have your priorities straight in the world. Very good.
Well, if it's possible to get your minds off Curt Schilling last night, and off more importantly tonight. Perhaps we can spend a bit of time this morning in the meanwhile with whatever spare neurons you have talking about recombinant DNA for a bit, OK? What we talked about last time was different ways to clone your gene based on its properties. We started off with cloning by complementation, right, the idea that if you took a library of clones, you would be able to put it into bacteria and select a bacterium whose phenotype had been restored by virtue of having the plasmid.
You would complement the defect. You'd find the clone you wanted because it complemented the defect. That's great if you can put it into an organism that has a defect. You can do it with bacteria. You can do that with yeast. It's harder to do with large organisms because you can't inject enough of them with different clones to be able to make that practical unless you're working in cell culture or some very small, fast growing organism.
We talked about being able to use a protein sequence, reverse translating that Lecture 17
Good morning. Good morning. I don't know about you, but I can't take too many more nights like this. I confess, I haven't gotten a thing done for so many nights in a row now, but what a game! How many of you saw the game? Excellent. Very good, very good. You have your priorities straight in the world. Very good.
Well, if it's possible to get your minds off Curt Schilling last night, and off more importantly tonight. Perhaps we can spend a bit of time this morning in the meanwhile with whatever spare neurons you have talking about recombinant DNA for a bit, OK? What we talked about last time was different ways to clone your gene based on its properties. We started off with cloning by complementation, right, the idea that if you took a library of clones, you would be able to put it into bacteria and select a bacterium whose phenotype had been restored by virtue of having the plasmid.
You would complement the defect. You'd find the clone you wanted because it complemented the defect. That's great if you can put it into an organism that has a defect. You can do it with bacteria. You can do that with yeast. It's harder to do with large organisms because you can't inject enough of them with different clones to be able to make that practical unless you're working in cell culture or some very small, fast growing organism.
We talked about being able to use a protein sequence, reverse translating that protein sequence in the computer from amino acid sequence to nucleotide sequence, and using the nucleotide sequence to design a probe to hybridize back to the genome. That works fine if you have a protein sequence.
But the last topic we talked about that I wanted to just touch on again this morning was suppose you were trying to clone the gene that causes a certain human disease, and you have no idea what the protein was. Then, you can't use its amino acid sequence because you don't have the protein. What can you possibly do when all you know is that you have a gene which causes a genetic defect that causes a disease? And I said you could clone it using the ideas of genetic mapping, position, the things that Sturtevant developed. And, I touched on it briefly, and I want to just touch on it a bit more because some people had some questions about it.
