Chasing the Dawn

Ch8: Strange Words Medicine Experiment

William Hart removed a lifting tube from the liquid nitrogen tank, then removed a cryopreservation tube from the lifting tube, and carefully put the lifting tube back because there were many cryopreservation tubes of other cells in the lifting tube. After William sealed the lock cover of the liquid nitrogen tank, he began a series of patient and meticulous operations.

He first put the cryopreservation tube in a water bath, thawed it quickly with 37c hot water, then changed the centrifuge tube and centrifuged it for five minutes, then sat down at the clean bench, baked the mouth of the centrifuge tube in an alcohol lamp for disinfection, discarded the waste liquid, added fresh culture medium with a pipette, and repeatedly blown and mixed...

Arthur Wenlock was watching carefully at the side and asked William for advice when he had any questions and learned a lot.

Like the speed control of the centrifuge, the suction and release control of the pipette, etc., these recovery operations will affect the survival rate and proliferation rate of cells if they are careless.

Finally, William injected the cell suspension into the culture dish, added an appropriate amount of culture medium, and then put it into the cell culture incubator at 37c and 5% CO2.

"Wait at least two days." William took off his mask and pushed his glasses. "I suggest you keep it for a week so that the tumor formation rate is guaranteed."

"Let's try a batch of two-day ones first." Arthur shrugged and said, "There was no way; there was not enough time." The system showed that his condition would worsen in 30 days. If it was true, he might even have difficulty getting out of bed and walking. He said again, "Brother, let's go out for a meal tonight. I'll treat you."

"No thanks." William waved his hand. I have to feed the mice medicine tonight. I'll treat them."

...

Arthur spent the next two days on campus. In addition to meeting up with friends, he read books and prepared for the make-up exam. If he didn't die but had to repeat the grade, it would be quite troublesome; second, he went to the laboratory building every day to take care of the cells and change the culture medium.

On the morning of the third day, William came to see it in person and said that the cells had grown barely enough. He helped with the subculture and preparation of cell suspension.

Arthur went to the school's experimental animal center, which is a large breeding base located a few hundred meters outside the school campus. It is very lonely, probably because it is filled with the stench of various animals.

He bought five nude mice, each costing more than 100 dollars, which is quite expensive. Fortunately, he still has 20,000 dollars in savings, but it won't last long.

Money is not enough when it is needed. Arthur regrets that he donated the money so early... He is still alive, but the money is gone.

Each of these nude mice is only the size of two or three fingers put together, and they have no hair. They are placed in a sterile box. Once they come into contact with the external bacterial environment, they will easily be infected and die. Therefore, the usual breeding and experimental processes need to be carried out in a barrier environment.

Grandville University's barrier environment laboratories are all in the experimental animal research center. This five-story yellow brick wall building stands next to the experimental building.

Arthur and William met in the lobby on the first floor of the research center and booked a small nude mouse room for one hour. This room is only 21 square meters and is divided into the front and back rooms. The front room has a laboratory table and instruments, and the back room is the breeding room.