Chapter 11

132 9 1
                                    

The Peony Hairclip

I made it clear last night that what I feel about Adam is only a foreign sensation for me that I chose not to explore. I forced myself not to allow my growing emotions distract me, and I meant to forget about it completely, without him knowing.

He's already content with his life right now, and I have no plans to spoil it.

What I need right now is a diversion to help me focus more on my OJT and to achieve my goal.

I'm doing this because of my family.

Family only.

"Girl, help me!" Sariah barged into laboratory room without minding the presence of other doctors and researchers inside the room.

I'm currently examining adult stem cells extracted from one of their patients' bone marrow since Dr. Manalastas, the doctor in charge of me for the day, wanted me to learn about how stem cells work as regenerative medicine in terms of body tissue and organ repair, congenital defects, and disease.

"Why? What's wrong?" I asked her while recording my observation on the reaction of the induced pluripotent stem cells in fibrosis.

"Paano kasi gumawa ng interpretation for DNA methylation? Kanina ko pa kasi inaaral pero hindi ko talaga mapiga sa utak ko." She said as she sits in front of me observing every move that I'm doing.

Yes, ganito kami kabusy ngayon at nasa first week palang naman kami. Hiwalay pa kaming inassign ni Dr. Evaristo ng mga tasks para daw masanay kami kung paano makipag-trabaho sa iba't ibang doctors. Even though I'm enjoying my work, I am nonetheless exhausted from it. Parang anytime, ako mahihilo sa pagod. Minsan kasi ay hindi na kami nakakapag-lunch dahil sa dami ng experiments na kailangan namin gawin. Most of the time, we're helping our seniors with the completion of their experiments.

Ang ibang pinapagawa naman samin ay hindi naituro sa CRSU kaya habang cinoconduct namin ang experiment ay, sineself-study namin dahil hindi pwedeng magkamali kami sa ginagawa namin. 1 mistake and damay na ang evaluation namin.

"Ano bang modification ang inapply mo? LC-MS, MSRE, Digestion-based?" Sunod-sunod na tanong ko sakanya.

Nag-isip muna siya ng ilang segundo para maalala kung anong modification procedure yung pinapagawa sakanya.

"Yung Methylation Sensitive Restriction Enzyme. Sabi kasi nila mas mabilis yung detection ng DNA methylation pattern kapag iyon ang ginamit ko."

Agad na kumunot ang mga kilay ko sa sinabi niya dahil masyadong advanced ang pinapagawa sakanya. Sinubukan ko pa din siyang tulungan kaya kinalkal ko sa utak ang mga natatandaan ko tungkol sa procedures ng MSRE.

"Once na yung DNA methylation is digested using the MSRE technique, pwede mong gamitin ang qPCR gamit ang mga primers na nagsusurround sa mismong sequence ng patterns. Tapos kunin mo ang methylation percentage using threshold cycles para mameasure both ang digested and undigested control DNA." Dahan-dahan kong paliwanag sakanya.

"Need ba talaga threshold cycle yung unit of measurement ang gamitin ko? Nahihirapan kasi ako doon baka hindi accurate yung makuha ko."

"Ayun kasi talaga ang ginagamit kapag MSRE, pero subukan mong tanungin yung in-charge sayo for clarification para matulungan ka din."

"Hay, nakakapagod na to. Ayaw ko na!" Biglaang pagreklamo niya sabay pagsipa sa hangin.

Ngumiti lang ako ng kaunti sakanya at ngayon naman ay susubukan ko pag-aralan kung paano gumagana ang stem cells sa mga neurodegenerative disorders. I find it interesting kasi unlike sa mga body tissues and organ reparation, iba kapag sa utak mismo ang nagkaroon ng problema.

When the Sky Meets the SeaTahanan ng mga kuwento. Tumuklas ngayon